Fig 4.

Extracellular region of Rv2272 bound and activated γδ T cells in vitro. (A) The predicted transmembrane region of Rv2272. (B) The IL-2 production of PT-transfected cells stimulated by every region of Rv2272. EP and IP1 could stimulate PT-transfected cells to produce IL-2 in a dose-dependent manner. The single asterisk denotes significant differences (P < 0.05). The double asterisks denote significant differences (P < 0.01). Data are the means from three independent experiments. (C) EP of Rv2272 induced the expansion of γδ T cells from pulmonary tuberculosis patients. The percentage of γδ T cells was measured 2 weeks later by FACS using FITC-αβ TCR (clone WT31; BD) and PE-γδ TCR (clone 11F2; BD). The percentage of γδ T cells is shown in the form of a histogram. Data are shown as the mean percentages of γδ T cells ± standard deviations from two independent experiments. (D) Detection of γδ T cell proliferation induced by EP with CCK-8 assay. Data are the means from three independent experiments.