Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| B. henselae | ATCC 49882 | ATCC |
| B. quintana Oklahoma | Human isolate | P. Brouqui |
| E. coli TOP10 | Host strain for cloning | Invitrogen |
| E. coli BL21Star(DE3) | Host strain for gene expression | Invitrogen |
| Plasmids | ||
| pCR4-TOPO | TA-cloning vector | Invitrogen |
| pET100D/TOPO | Expression vector | Invitrogen |
| pHbpA | pET100D/TOPO containing hbpA | This study |
| pHbpB | pET100D/TOPO containing hbpB | This study |
| pHbpC | pET100D/TOPO containing hbpC | This study |
| pHbpD | pET100D/TOPO containing hbpD | This study |
| pHbpE | pET100D/TOPO containing hbpE | This study |
| pHbpB1 | pET100D/TOPO containing hbpB (1–354) | This study |
| pHbpB2 | pET100D/TOPO containing hbpB (265–660) | This study |
| pHbpB3 | pET100D/TOPO containing hbpB (601–900) | This study |
| pHbpB4 | pET100D/TOPO containing hbpB (901–1362) | This study |
| pHbpD1 | pET100D/TOPO containing hbpD (1–300) | This study |
| pHbpD2 | pET100D/TOPO containing hbpD (151–450) | This study |
| pHbpD3 | pET100D/TOPO containing hbpD (301–600) | This study |
| pHbpD4 | pET100D/TOPO containing hbpD (451–750) | This study |
| pHbpD5 | pET100D/TOPO containing hbpD (601–885) | This study |
a
Numbers indicate the positions in the nucleotide sequence of the appropriate gene.