Table 2.
Primers for construction of hemin-binding proteins in Bartonella quintana
| Construct | Sequence (5′→3′) |
|
|---|---|---|
| Forward primera | Reverse primer | |
| HbpA | CACCATGAATATAAAATCTTTAATAACG | TTAGAATTTATAAGCTACACCAAAACGG |
| HbpB | CACCATGAATACGAAACGTTTAATAAC | TTAGAATTTATAAGCTACACCAATACG |
| HbpC | CACCATGAATATGAAATGGTTAATAAC | TTAAAATTTGTAAGCCACACCAACGC |
| HbpD | CACCATGGCTAAAAAATATTTAATCAC | TTAAAATTTGTACGCTACACCAACACGG |
| HbpE | CACCATGAATATGAAATTTATAATAGG | TTAGAATTTGTAAGCTACACCCACACG |
| HbpB1 | CACC ATG AATACGAAACGTTTAATA | TTATGTGTATTTTTGTCCAGACCA |
| HbpB2 | CACCGGTCTTTACGCCGGTTCTAAT | TTATGTTAATGATGGTCGGGCTAC |
| HbpB3 | CACCCCAGCAGCACCAGCGTCGGTA | TTACTCACCTTGTGCACTCCGTCC |
| HbpB4 | CACCCAAACGGTGAATGAAAAAAGT | TTAGAATTTATAAGCTACACCAAT |
| HbpD1 | CACCATGGCTAAAAAATATTTAATC | TTAGAAATCAAAATTGAAACCGCC |
| HbpD2 | CACCATAGGTAATTTTTCAAGTAAG | TTACCCTGGAATCTCTATCCCTGA |
| HbpD3 | CACCGGCAAGGGTTTTGTTATAGGT | TTACAAGCGCTCCCCAGAAAAACC |
| HbpD4 | CACCACAGAGTTTCCAGGAGGGGTA | TTAAAAATCAACACCGCCTCCAAG |
| HbpD5 | CACCATGCCTTATATTGCTGGAGGT | TTAAAATTTGTACGCTACACCAAC |
a
CACC at the 5′ end is a sequence added to allow pET directional cloning.