Fig 1.

AD169_IE2-YFP-infected cells express IE2-YFP as an immediate-early protein. (A) Diagram of the recombinant AD169_IE2-YFP virus. CMV unique long (UL) and unique short (US) regions of the genome, the orientation of the UL121-124 genes, and the UL122-YFP chimera are indicated. (B) AD169_GFP-infected (lanes 1 to 12) and AD169_IE2-YFP-infected (lanes 13 to 24) (MOI of 1) MRC5 cells were harvested at between 0 and 22 hpi and subjected to immunoblot analysis for IE1 and IE2 polypeptides and actin protein. The respective polypeptides and relative molecular mass markers are indicated. (C) Mock- and AD169_IE2-YFP-infected MRC5 cells (MOI of 5) were stained with Hoechst reagent and analyzed by using confocal microscopy (magnification, ×20). The Hoechst reagent and IE2-YFP signal localized exclusively to the nucleus.