Fig 6.
Analysis of the ability of sera from different donors to neutralize AD169IE2-YFP infection. (A) Anti-CMV IgG and IgM levels from sera of five human donors were determined by using a commercial ELISA-based anti-CMV kit. Cutoff values for CMV-positive and CMV-negative donors, as recommended by the manufacturer, are indicated with dashed lines. Student's t test showed significance of IgG and IgM levels in all samples compared to the CMV-negative control (*, P < 0.05). (B) AD169IE2-YFP-infected MRC5 cells (MOI of 5) preincubated with serum (1:100 dilution) from all five donors were analyzed (14 hpi) by using a microplate cytometer to determine YFP-positive cells. The percentage of infected cells was calculated by using AD169IE2-YFP-infected cells as 100%. Student's t test showed significance in all samples compared to the CMV-negative control (*, P < 0.05). Error bars represent standard errors of means.