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Both deletion and substitution emerged in the untranslated regions (UTR) during in vitro serial passage. One guanine deletion were identified at nt 119 in the 5′ UTR of JXA1 from passages 140 to 170 (A), while two substitutions (T115C and A123G) were found in the 3′ UTR of JXA1 from passages 80 to 170 (B). Both the deletion and substitutions are highlighted in black characters on a gray background.
