Table 1.

Trial groups and treatments^a

Trial	Trial group	No. of animals/group	Vaccination protocol
			Proteins	Adjuvant	Vaccination weeks
1	Rectal	3	100 μg intimin, 100 μg ovalbumin	50 μg CTB	0, 3, 6
	SQ	3	33 μg intimin, 33 μg ovalbumin	1.5 ml Freund's	0, 3, 6
2	Rectal	3	100 μg intimin, 100 μg ovalbumin	5 mg R848	0, 3, 6
	SQ	3	33 μg intimin, 33 μg ovalbumin	50 μg R848	0, 3, 6
	Adjuvant (SQ)	3	None	50 μg R848	0, 3, 6
3	Rectal	5	100 μg and 300 μg intimin, 100 μg and 300 μg ovalbumin	5 mg R848	0, 8, 11
	SQ	5	100 μg intimin, 100 μg ovalbumin	50 μg R848	0, 8, 11

^aSummary of treatments for each trial group. In trial 1, complete Freund's adjuvant was used in the first immunization, and incomplete adjuvant was used for subsequent immunizations. In the rectal groups of trial 3, all animals received one subcutaneous immunization (100 μg protein), and subsequent immunizations were mucosal applications of 300 μg of each protein.