Table 2.
Serum anti-hemagglutinin antibody in chickens immunized with pCAG-HA DNA vaccine carrying the hemagglutinin gene of avian influenza virus strain A/Hanoi/30408/2005 (H5N1) as measured by a competitive ELISA
| Vaccine dose (μg) | + Electroporation |
− Electroporation |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vaccinated birds |
Seroconversion during the course of expt |
Vaccinated birds |
Seroconversion during the course of expt |
|||||||||
| Total no. | Median % inhibition | Range of inhibition (%) | No. of seropositive birds/total no. immunized | Earliest detection, (avg dpv)a | Antibody persistence (avg no. of days)b | Total no. | Median % inhibition | Range of inhibition (%) | No. of seropositive birds/total no. immunized | Earliest detection, (avg dpv)a | Antibody persistence (avg no. of days) | |
| 0 | 5 | 3 | 0–9 | 0/5 | Not detected | 0 | 10 | 0 | 0 | 0/10 | Not detected | 0 |
| 10 | 13 | 48 | 3–99 | 6/13 | 42 | 10 | 13 | 9 | 0–36 | 0/13 | Not detected | 0 |
| 100 | 12 | 85 | 29–101 | 11/12 | 31 | 26 | 12 | 12 | 0–47 | 0/12 | Not detected | 0 |
| 250 | 5 | 96 | 58–99 | 5/5 | 29 | 26 | 5 | 17 | 1–24 | 0/5 | Not detected | 0 |
a
Not detected: none of the chickens tested positive at any of the sampling times. Serum with a cELISA inhibition value of ≥50% was considered positive.
b
The study design allowed only for measuring antibody persistence to a maximum of 43 days before termination of experiment.