Fig 4.

Carbohydrate antigen specificity of LAM-CRT-induced Abs. Groups of BALB/c mice (A) and nude mice (B) were s.c. injected with LAM-CRT (in PBS; 100 μg/mouse, 6 per group) and boosted with 50 μg of the same Ag preparations 2 weeks later. Antisera collected on day 28 from each group were pooled, diluted 1/200, and dispensed in triplicate wells that had been precoated with dextran, pustulan, linear β-1,3-glucan, laminarin, mannan, alginic acid, and heparin for ELISAs. HRP-conjugated goat anti-mouse IgG was employed as a detection Ab followed by development with OPD. Wells not precoated with any Ags (None) were included as a control. The results are expressed as OD₄₉₂ with SD. ***, P < 0.001.