Fig 6.

Vaccination with LAM-CRT confers protective immunity against C. albicans infection. BALB/c mice (8 per group) were immunized twice with LAM-CRT or rCRT/39–272 followed by a lethal i.v. challenge with C. albicans 28 days later. (A) The mice were observed daily for up to 40 days, and percent survival was calculated. (B) Groups of BALB/c mice (4/group) that had been similarly immunized were sacrificed for kidney analyses 2 days after a lethal challenge with C. albicans. Viable yeast cells in the kidneys were individually quantitated using the plate dilution method, and the results are expressed as mean CFU/kidney (of 4 kidneys) ± SD. (C) C. albicans cells were cultured overnight in the presence of NMS (open bars)- or LAM-CRT (filled black bars)- or rCRT/39–272 (filled gray bars)-induced antisera. Viable C. albicans cells in the cultures were then quantitated, and the results were calculated using the “NMS” wells as a reference and are expressed as mean percent growth inhibition ± SD of the results determined with triplicate wells. Data are from three independent immunization experiments. **, P < 0.01; ***, P < 0.001.