Fig 7.

Immunogold EM analysis using anti-Sto4p (A to C and E) and anti-Sto8p (D) Abs. (A) Gold label is scattered in the CVC between the membrane labyrinth of the smooth spongiome (ss) close to the radial canals (rc) but not in the decorated spongiome (ds). With both Abs, labeling in microdomains occurs close to the cell membrane. (B to D) Label close to or at alveolar sacs (as) is particularly evident where cells are cut tangentially. Note the absence of label on a cilium (ci). (E) Label is enriched on a population of small vesicles close to the food vacuole and on the vacuole membrane itself. For further details, see Materials and Methods. Scale bars, 0.1 μm.