Fig 8.

Germination-specific expression of cda1 revealed by a B. cinerea cda1-GFP promoter reporter strain. Conidia were germinated on a polypropylene surface in minimal medium with 10 mM fructose. (A) Time course of germination and appearance of GFP fluorescence. The means of three experiments are shown, with standard deviations (n > 150). (B) Time-lapse microscopy of germinating conidia during a 3.5-h observation period. Conidia that started to show fluorescence before germ tube outgrowth are circled in red. A nonfluorescent spore that did not germinate during the observation period is circled in blue.