Table 2.
Induction of [URE3] in Candida glabrataa
| Strain | URA3 plasmid | HIS3 plasmid | No. Ade+/106 cells in expt: |
||||
|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |||
| HCg25 CgURE2 | CgURE2 (pH1259) | Vector (pH1286) | <1 | <1 | <1 | <1 | <1 |
| CgURE2 (pH1259) | CgURE2 aa 1–100 (pH1287) | <1 | <1 | <1 | <1 | <1 | |
| HCg32 URE2albicans | CaURE2 (pH1258) | Vector (pH1286) | <1 | <1 | 12 | 1 | 1 |
| CaURE2 (pH1258) | CaURE2 aa 1–89 (pH1288) | 36 | 15 | 30 | 24 | 95 | |
a
Cells were grown on medium without adenine, adjusted to pH 5.6, and contained both ammonium and glutamate as nitrogen sources. Colonies were counted after 5 days at 30°C. No histidine was added, as this seemed to increase the background.