Table 3.
Infection of S. cerevisiae with [URE3albicans] from extracts of C. glabrata strainsa
| Protein extract source | Ade+ ScBY302 of 48 tested | Ade+ clone no. | No. Ade+/color on YES after growth on: |
|
|---|---|---|---|---|
| YPAD | YPAD + 3 mM guanidine | |||
| YHE1161 | 39 | |||
| 19 | 12/white | 0/red | ||
| 20 | 12/white | 0/red | ||
| 21 | 12/white | 0/red | ||
| 22 | 12/white | 0/red | ||
| H2O | 0 | |||
| HCg32 | ||||
| 1 | 8 | |||
| 11 | 11/red | 0/red | ||
| 12 | 12/red | 0/red | ||
| 13 | 12/red | 0/red | ||
| 14 | 12/red | 0/red | ||
| 15 | 12/red | 0/red | ||
| 16 | 12/red | 0/red | ||
| 17 | 12/red | 0/red | ||
| 18 | 12/red | 0/red | ||
| 2 | 4 | |||
| 8 | 10/red | 0/red | ||
| 9 | 10/red | 0/red | ||
| 10 | 7/white | 7/white | ||
| 3 | 0 | |||
| 4 | 0 | |||
| 5 | 1 | |||
| 7 | 12/white | 12/white | ||
| 6 | 6 | |||
| 1 | 12/red | 0/red | ||
| 2 | 12/red | 0/red | ||
| 4 | 12/red | 0/red | ||
| 5 | 12/red | 0/red | ||
| 6 | 12/red | 0/red | ||
a
BY302 cells were transformed with the indicated extract and the LEU2 plasmid pRS425 and plated on −Leu plates containing 5 mg/liter adenine. Forty-eight transformant colonies from each plate were picked and tested for growth on SD+H+W (−Ade −Leu). The number of colonies growing on the indicated plates is shown. The indicated Ade+ clones were then streaked for single colonies on YPAD or YPAD with 3 mM guanidine, and 12 were tested from each plate for growth on −Ade medium (SD+H+W+L) and color on YES. The number of Ade+ clones and their color on YES is shown.