Table 4.
Protein extract | S. cerevisiae BY302 Ade+ clone no. and color on YES | No. of Ade+ cytoductants/total |
---|---|---|
YHE1161 | 19; white | 28/28 |
20; white | 30/30 | |
HCg32 | ||
1 | 11; red | 26/28 |
12; red | 18/18 | |
2 | 8; red | 2/44 |
9; red | 27/43 | |
6 | 1; red | 41/42 |
2; red | 26/26 |
The cytoduction recipient was YHE1364, isogenic with BY302, but with different markers. Cytoductants were tested for ability to grow without adenine. Numbers are expressed as no. of Ade+ cytoductants out of the total number of cytoductants assayed.