Fig 4.

Spontaneous and GlcNAc-induced switching phenotypes of CAF-1 and HIR mutants. (A) Quantitative switching assays were performed to determine the spontaneous and N-acetylglucosamine (GlcNAc)-induced switching frequencies for each mutant. Values are average percentages of total colonies displaying a sector or colony that switched phenotypes ± the standard deviations for the independent tests done for each strain. A total of 3 or 4 independent tests were done on different days for each strain. Switching assays were performed on synthetic complete agar plates left at room temperature for 1 to 2 weeks, depending on the strain used. (B) White- and opaque-cell phenotypes of indicated strains. The opaque cells express a WOR1p-GFP reporter gene, whereas white cells lack fluorescence (arrowheads). White and opaque cells were collected from white and opaque sectors taken from SC-GlcNAc-containing plates. Cells were mixed together and imaged. Bar, 10 μm. Images were obtained by differential interference contrast microscopy (DIC).