Table 1.
Strains used in this study
| Strain | Descriptiona | Genotype | Reference or source |
|---|---|---|---|
| JYC5 | WT | MTLa/a ura3::imm434/ura3::imm434 | 31 |
| HLY4125 | hir1Δ/Δ | MTLa/a hir1Δ::FRT/hir1Δ::FRT | This study |
| HLY4126 | cac2Δ/Δ | MTLa/a cac2Δ::FRT/cac2Δ::FRT | This study |
| HLY4127 | hir1Δ/HIR1 | MTLa/a hir1Δ::FRT/HIR1::FRT | This study |
| HLY4128 | cac2Δ/CAC2 | MTLa/a cac2Δ::FRT/CAC2::FRT | This study |
| HLY4129 | hir1Δ/Δ cac2Δ/Δ | MTLa/a hir1Δ::FRT/hir1Δ::FRT cac2Δ::FRT/cac2Δ::FRT | This study |
| HLY3997 | rtt109Δ/Δ | MTLα/α rtt109Δ::FRT/rtt109Δ::FRT | 37 |
| HLY4130 | rtt109Δ/Δ hir1Δ/Δ | MTLα/α rtt109Δ::FRT/rtt109Δ::FRT hir1Δ::FRT/hir1Δ::FRT | This study |
| HLY4131 | rtt109Δ/Δ cac2Δ/Δ | MTLα/α rtt109Δ::FRT/rtt109Δ::FRT cac2Δ::FRT/cac2Δ::FRT | This study |
| HLY4132 | rtt109Δ/Δ hir1Δ/HIR1 | MTLα/α rtt109Δ::FRT/rtt109Δ::FRT hir1Δ::FRT/HIR1::FRT | This study |
| HLY4133 | rtt109Δ/Δ cac2Δ/CAC2 | MTLα/α rtt109Δ::FRT/rtt109Δ::FRT cac2Δ::FRT/CAC2::FRT | This study |
| HLY3555 | WT | MTLa/a ura3::imm434/ura3::imm434 | 31 |
| +WOR1p-GFP | WOR1/PWOR1-GFP-URA3-WOR1 | ||
| HLY4134 | hir1Δ/Δ | MTLa/a hir1Δ::FRT/hir1Δ::FRT | This study |
| +WOR1p-GFP | WOR1/PWOR1-GFP-URA3-WOR1 | ||
| HLY4135 | cac2Δ/Δ | MTLa/a cac2Δ::FRT/cac2Δ::FRT | This study |
| +WOR1p-GFP | WOR1/PWOR1-GFP-URA3-WOR1 | ||
| HLY4136 | hir1Δ/HIR1 | MTLa/a hir1Δ::FRT/HIR1::FRT | This study |
| +WOR1p-GFP | WOR1/PWOR1-GFP-URA3-WOR1 | ||
| HLY4137 | cac2Δ/CAC2 | MTLa/a cac2Δ::FRT/CAC2::FRT | This study |
| +WOR1p-GFP | WOR1/PWOR1-GFP-URA3-WOR1 | ||
| HLY4138 | hir1Δ/Δ cac2Δ/Δ | MTLa/a hir1Δ::FRT/hir1Δ::FRT cac2Δ::FRT/cac2Δ::FRT | This study |
| +WOR1p-GFP | WOR1/PWOR1-GFP-SAT1-WOR1 | ||
| HLY3998 | rtt109Δ/Δ | MTLα/α rtt109Δ::FRT/rtt109Δ::FRT | 37 |
| +WOR1p-GFP | WOR1/PWOR1-GFP-SAT1-WOR1 | ||
| HLY4139 | rtt109Δ/Δ hir1Δ/Δ | MTLα/α rtt109Δ::FRT/rtt109Δ::FRT hir1Δ::FRT/hir1Δ::FRT | This study |
| +WOR1p-GFP | WOR1/PWOR1-GFP-SAT1-WOR1 | ||
| HLY4140 | rtt109Δ/Δ cac2Δ/Δ | MTLα/α rtt109Δ::FRT/rtt109Δ::FRT cac2Δ::FRT/cac2Δ::FRT | This study |
| +WOR1p-GFP | WOR1/PWOR1-GFP-SAT1-WOR1 | ||
| HLY4141 | rtt109Δ/Δ hir1Δ/HIR1 | MTLα/α rtt109Δ::FRT/rtt109Δ::FRT hir1Δ::FRT/HIR1::FRT | This study |
| +WOR1p-GFP | WOR1/PWOR1-GFP-SAT1-WOR1 | ||
| HLY4142 | rtt109Δ/Δ cac2Δ/CAC2 | MTLα/α rtt109Δ::FRT/rtt109Δ::FRT cac2Δ::FRT/CAC2::FRT | This study |
| +WOR1p-GFP | WOR1/PWOR1-GFP-SAT1-WOR1 | ||
| HLY4055 | WT | MTLa/a ura3::imm434/ura3::imm434 | 37 |
| +WOR1p-GFP | WOR1/PWOR1-GFP-URA3-WOR1 | ||
| +MAL2-WOR1-3×HA | ADH1/adh1::PMAL2-WOR1-3XHA-SAT1 | ||
| HLY4143 | hir1Δ/Δ | MTLa/a hir1Δ::FRT/hir1Δ::FRT | This study |
| +WOR1p-GFP | WOR1/PWOR1-GFP-URA3-WOR1 | ||
| +MAL2-WOR1-3×HA | ADH1/adh1::PMAL2-WOR1-3XHA-SAT1 | ||
| HLY4144 | cac2Δ/Δ | MTLa/a cac2Δ::FRT/cac2Δ::FRT | This study |
| +WOR1p-GFP | WOR1/PWOR1-GFP-URA3-WOR1 | ||
| +MAL2-WOR1-3×HA | ADH1/adh1::PMAL2-WOR1-3XHA-SAT1 | ||
| HLY4145 | hir1Δ/Δ cac2Δ/Δ | MTLa/a hir1Δ::FRT/hir1Δ::FRT cac2Δ::FRT/cac2Δ::FRT | This study |
| +WOR1p-GFP | WOR1/PWOR1-GFP-URA3-WOR1 | ||
| +MAL2-WOR1-3×HA | ADH1/adh1::PMAL2-WOR1-3XHA-SAT1 | ||
| HLY4057 | rtt109Δ/Δ | MTLα/α rtt109Δ::FRT/rtt109Δ::FRT | 37 |
| +MAL2-WOR1-3×HA | ADH1/adh1::PMAL2-WOR1-3XHA-SAT1 | ||
| HLY4146 | rtt109Δ/Δ hir1Δ/Δ | MTLα/α rtt109Δ::FRT/rtt109Δ::FRT hir1Δ::FRT/hir1Δ::FRT | This study |
| +MAL2-WOR1-3×HA | ADH1/adh1::PMAL2-WOR1-3×HA-SAT1 | ||
| HLY4147 | rtt109Δ/Δ cac2Δ/Δ | MTLα/α rtt109Δ::FRT/rtt109Δ::FRT cac2Δ::FRT/cac2Δ::FRT | This sudy |
| +MAL2-WOR1-3×HA | ADH1/adh1::PMAL2-WOR1-3XHA-SAT1 | This study | |
| HLY3993 | HST3/hst3 | MTLα/α ade2/ade2 ura3::ADE2/ura3::ADE2 HST3/hst3::FRT | 37 |
| JMR114 | hog1Δ/Δ | hog1Δ::ARG4/hog1Δ::URA3 | 48 |
a
WT, wild type.