Table 2.
Wor1 can bypass the requirement for CAC2, HIR1, and RTT109
| Genotype | White→opaque switching on YEP-maltosea |
|
|---|---|---|
| % | n | |
| WT | ||
| Control | 0.11 ± 0.25 | 836 |
| +MAL2p-WOR1-3×HA | 99.80 ± 0.40 | 503 |
| hir1Δ/Δ | ||
| Control | 0.11 ± 0.22 | 862 |
| +MAL2p-WOR1-3×HA | 100 ± 0.00 | 580 |
| cac2Δ/Δ | ||
| Control | 1.71 ± 0.69 | 708 |
| +MAL2p-WOR1-3×HA | 99.38 ± 0.51 | 958 |
| hir1Δ/Δ cac2Δ/Δ | ||
| Control | 0.35 ± 0.40 | 858 |
| +MAL2p-WOR1-3×HA | 100.00 ± 0.00 | 736 |
| rtt109Δ/Δ | ||
| Control | 0.06 ± 0.29 | 1,272 |
| +MAL2p-WOR1-3×HA | 99.00 ± 0.15 | 1,432 |
| rtt109Δ/Δ hir1Δ/Δ | ||
| Control | 0.70 ± 0.72 | 1,032 |
| +MAL2p-WOR1-3×HA | 97.69 ± 0.72 | 1,195 |
| rtt109Δ/Δ cac2Δ/Δ | ||
| Control | 1.56 ± 0.74 | 1,079 |
| +MAL2p-WOR1-3×HA | 98.10 ± 1.32 | 1,043 |
a
White cells carrying a MAL2p-WOR1-3×HA transgene or control vector (GFP) were plated on YEP–2% maltose test plates and scored for opaque-cell formation after 1 to 2 weeks growth at room temperature. Values are average percentages of total colonies (n) displaying a sector that switched phenotypes ± standard deviations for 2 independent tests done on different days for each strain.