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. 2013 Jan 15;2(1):58–65. doi: 10.7774/cevr.2013.2.1.58

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© Korean Vaccine Society.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — Cross-reactivity of the pep27 mutant antisera with heterologous strains. Strains grown in THY broth were lysed, and cell lysates (10 µg/mL) of each pneumococcal strain were adsorbed onto titer plates. Antisera raised against the live pep27 mutant (THpep27) in CD1 mice after three i.n. immunizations was used as antisera for titer determination. The antigens used in this study included: D39 (type 2), EF3030 (type 19F), TIGR4 (type 4), BG7322 (type 6B), WU2 (type 3), R6 (non-encapsulated type 2). Student's t-test is indicated as follows: ^**p<0.01, ^***p < 0.001.