Skip to main content
. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: J Neurochem. 2012 Nov;123(0 2):125–137. doi: 10.1111/j.1471-4159.2012.07951.x

Figure 3. LPS preconditioning increases mitochondrial functional capacity and axonal movement.

Figure 3

Cellular ATP content (A) and citrate synthase activity (B) were assessed in lysates obtained from neuronal cultures exposed to 1ug/mL LPS at the given timepoints. Data were obtained from 3 separate cultures and statistically analyzed using ANOVA and post hoc Bonferroni/Dunn tests. *p<0.05, **p<0.01 versus controls (0). C. Mitochondrial respiration was measured in live cultures using the Seahorse analyzer. Cultures were treated with medium as a vehicle control, FCCP or oligomycin at the indicated time and the rate of oxygen consumption was recorded. D. The data were normalized in vehicle controls and statistically compared using two-tail Student’s t-test, *p<0.05 versus vehicle. E-F. Mitochondrial movement along axonal and dendritic regions was assessed in live cultures under time-lapse imaging. Data were from 3 separate cultures and each experiment was done in quadruplicate wells. *p<0.05 between the indicated groups; n.s., not significant.