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. 2013 Apr 15;24(8):1122–1133. doi: 10.1091/mbc.E12-11-0817

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© 2013 Tang et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Mitotic spindles are morphologically normal in ndc80-NH12, yet chromosome missegregation is induced. (A) Time-lapse fluorescence montages of the spindle microtubule (GFP-Atb2, green, middle), kinetochore (Mis6-2mRFP, two copies of monomeric RFP, red, bottom), and the SPB (Cut12-CFP, shown in the merged images on the top) are shown in wild-type cells (left) and type I (middle) and type II (right) ndc80-NH12 cells incubated at 36°C for 1 h. Representative images of each strain are shown. Unlike wild-type cells, chromosomes (kinetochores) segregate unequally in ndc80-NH12 cells. Overall spindle microtubule structures (0–6 min), including their intensities and morphologies, are indistinguishable between wild-type and ndc80-NH12 cells. Note that GFP-Atb2 signals in wild-type (left) and type II (right) cells became dim after 6 min, ascribable to fluorescence bleaching. Scale bars, 5 μm.