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. 2013 Apr 15;24(8):1122–1133. doi: 10.1091/mbc.E12-11-0817

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© 2013 Tang et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — ndc80-NH12 genetically behaves like the alp7 or alp14 mutant, and the loop is a composite platform for Dis1 and Alp7–Alp14. (A) Tetrad analysis. Tetrad dissection was performed between a dis1-deletion strain (dis1::ura4⁺) and ndc80-NH12 (marked with kan). Four representative tetrads (tetratype) are shown. Far right, assigned genotypes of each segregant (Δdis1 = dis1::ura4⁺, NH12 = ndc80-NH12, and wild type [WT]). In all four tetrads, double mutants (red, predicted based on Mendelian segregation of tetrads) were inviable. (B) Summary of genetic interactions. Solid lines, synthetically lethal; dotted lines, viable. (C) Intragenic complementation between the two loop mutants. Indicated diploid strains were constructed, and spot tests were performed at various temperatures. Note that a heterozygous diploid (ndc80-NH12/ndc80-21) ameliorated growth at 35 and 36°C compared with each homozygous diploid.