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. 2013 Apr 15;24(8):1153–1162. doi: 10.1091/mbc.E12-08-0607

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© 2013 Fu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Gp78 ubiquitin ligase activity induces mitochondrial fission. (A) Cos7 cells were cotransfected with pOct-dsRed and empty vector, Flag-gp78, or Flag-RINGmut, and 3D projections of mitochondrial pOct-dsRed fluorescence were generated from confocal stacks. (B) Bar graphs show the quantification of volume (μm³) and shape (percentage of mitochondria with an x/y-axis ratio of >2) of mitochondria from individual 3D projections and speed (μm/s) of individual mitochondria from 4D movies (mean ± SEM, 15–20 cells/experiment, n = 3; *p < 0.01, **p < 0.0001).