TABLE 1:

Kinetic parameters for the hydrolysis of H-Asp-NHMec and H-Glu-NHMec by DNPP-1.

Substrate	Km (10−3 mM)	kcat (s−1)	kcat/Km (s−1 M−1)
H-Asp-NHMec	13.58	0.136	10,330.6
H-Glu-NHMec	8.854	0.134	15,324.1

Kinetic parameters were obtained at pH 7.5, as described in Materials and Methods. Each value represents the mean of two independent experiments.