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. 2013 Apr 15;24(8):1196–1207. doi: 10.1091/mbc.E12-12-0852

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© 2013 Boulant et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Characteristics of clathrin-coated pits associated with entry of MRV virion and ISVP particles at the apical surface of polarized MDCK cells. Data were acquired by 4D spinning-disk confocal microscopy, as indicated for Figure 5. (A) Kinetic intensity profiles of single, representative coated pits: one empty, that is, not containing a fluorescent virus particle; one containing a fluorescent virion; and one containing a fluorescent ISVP. The AP2-GFP fluorescence intensity for each time point has been normalized to the maximum AP2-GFP fluorescence intensity reached during formation of the clathrin-coated pit in each example. (B) Scatter plot of the lifetimes of coated pits lacking or containing an MRV particle. Data are shown as the mean value ± SD from three cells for pits with each type of cargo; n = number of pits analyzed. Statistical significance values for the observed differences in pit lifetimes are shown. (C) Scatter plot of the maximum AP2-GFP fluorescence intensities of coated pits lacking or containing an MRV particle. The maximum fluorescence intensity of each pit during the course of uptake has been normalized to the average maximum fluorescence intensity of the empty pits. Data are shown as the mean value ± SD from three cells for pits with each type of cargo; n = number of pits analyzed. No statistically significant differences were found.