Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Mar 12;12:91. doi: 10.1186/1475-2875-12-91

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013 Chaianantakul et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

[³H]-FdUMP binding of crude extracts and partially purified proteins. Crude extracts and partially purified proteins from plasmids encoding pfDHFR co-transformed with pfJRTS mutants with truncated JR (panels A and B, respectively), and plasmids encoding pAC28 co-transformed with pfDHFR-TS with truncated JR (panel C and D, respectively) were labeled with [³H]-FdUMP. For panels A and B: lane 1, pfDHFR-TS (3D7); lane 2, pfJRTS△232-235 co-expressed with pAC28-pfDHFR; lane 3, pfJRTS△232-251 co-expressed with pAC28-pfDHFR; lane 4, pfJRTS△232-265 co-expressed with pAC28-pfDHFR; lane 5, pfJRTS△232-271 co-expressed with pAC28-pfDHFR; lane 6, pfJRTS△232-274co-expressed with pAC28-pfDHFR; lane 7, pfJRTS△232-276 co-expressed with pAC28-pfDHFR; lane 8, pfJRTS△232-277 co-expressed with pAC28-pfDHFR; lane 9, pfJRTS△232-299 co-expressed with pAC28-pfDHFR. For panels C and D: lane 1, pfDHFR-TS (3D7); lane 2, pfDHFR-TS△229-265; lane 3, pfDHFR-TS△229-271; lane 4, pfDHFR-TS△229-274; lane 5, pfDHFR-TS△229-275; lane 6, pfDHFR-TS△229-276; lane 7, pfDHFR-TS△229-277993. M is the molecular weight standard markers. (I) Coomassie blue stained SDS-PAGE, (II) autoradiogram of the SDS gel.