Figure 6.
Escherichia coli two-hybrid showing interaction between pfDHFR and pfTS domains.Escherichia coli BacterioMatch™ two-hybrid system (Strategene) was employed for the study of the domain-domain interaction between P. falciparum DHFR and TS domains. The gene coding for pfDHFR was cloned into the bait plasmid pBT, whereas the gene for pfJRTS was cloned into the target plasmid pTRG. The two recombinant plasmids were transformed into the E. coli XL1-blue MRF’ reporter strain. Positive control is E. coli XL1-blue MRF’ co-transformed with pBT-LDF2 and pTRG-Gal11p provided by the manufacturer. Negative control is E. coli XL1-blue MRF’. Agar plate on the left is LB-kanamycin (LB-K) and that on the right is LB- carbenicillin, tetracycline, chloramphenical and kanamycin (LB-CTCK).