Table 2.
Primers used for PCR diagnostic
| Target Species | Target gene | Primer name | Primer sequence (5’-3’) | Product size (kb) | Temp.*(°C) | Refs.† |
|---|---|---|---|---|---|---|
|
Host |
CytB rRNA |
CBJ-10933 |
TATGTACTACCATGAGGACAAATATC |
0.5 |
45 |
[42] |
|
(Curculio sp.) |
|
CBN-11367 |
ATTACACCTCCTAATTTATTAGGAAT |
|
|
[42] |
|
Eubacterial Universal Primers |
16S rRNA |
008For |
AGA GTT TGA TCA TGG CTC AG |
1.5 |
53 |
[43] |
| |
|
1487Rev |
TAC CTT GTT ACG ACT TCA CC |
|
|
(**) |
|
Candidatus Curculioniphilus buchneri |
16S rRNA |
16S-F |
AGAGATCTGGAGGAATATCA |
0.4 |
52 |
(*) |
| |
|
16S-R |
CACTAAAGCATCTCTGCTAAAT |
|
|
(*) |
| |
GroEL |
GroEL 2 F |
ATG GGB GCT CAA ATG GTK AAA |
0.9 |
55 |
[38] |
| |
|
GroEL 2R |
CTCTTTCATTTCAACTTCNGTBGCA |
|
|
[38] |
|
Wolbachia |
16S rRNA |
W-Spec F |
CATACCTATTCGAAGGGATAG |
0.4 |
60 |
[44] |
| |
|
W-Spec R |
AGCTTCGAGTGAAACCAATTC |
|
|
[44] |
|
Rickettsia |
16S rRNA |
RbF |
GCTCAGAACGAACGCTATC |
0.9 |
58 |
[12] |
| |
|
RbR |
GAAGGAAAGCATCTCTGC |
|
|
[12] |
|
Spiroplasma |
ITS and 16S rRNA |
Spixo-16S F |
TTAGGGGCTCAACCCCTAACC |
0.8 |
52 |
[45] |
| |
|
Spixo-16S R |
TCTGGCATTGCCAACTCTC |
|
|
[45] |
|
Sodalis |
16S rRNA |
Sodalis 370 F |
CGRTRGCGTTAAYAGCGC |
0.2 |
55 |
[38] |
| 16SSod590R | AACAGACCGCCTGCGTACG | [38] |
*Annealing temperature. †References: (*) this study; (**) nucleotides numbering from Escherichia coli, GenBank accession number J01859.