FIGURE 2. Effects of oligoamines on transactivation of ERα.

The cells were cultured in phenol red-free Dulbecco’s modified Eagle’s medium supplemented with 5% charcoal dextran-stripped fetal bovine serum and treated with 10 nM 17 β-estradiol (E₂). A, MCF-7 and T47D cells were treated with 10 μM of the specific oligoamine for 96 h in MCF-7 and 24 h in T47D cells. Cytoplasmic or nuclear protein was extracted for immunoblotting with anti-ERα and ERβ antibodies. Actin protein was blotted as a loading control for cytoplasmic extracts and proliferating cell nuclear antigen (PCNA) was used as a loading control for nuclear extracts. B, MCF-7and T47D cells were co-transfected with ERE-tk-luciferase and CMV-β-galactosidase. Reporter gene activities were measured after 24 h of oligoamine treatment for T47D cells and 96 h treatment for MCF-7 cells. The experiments were completed three times, and each measurement was taken in triplicate. *, p < 0.05, statistically significant differences using Student’s t test between control cells (treated with E₂ only) and cells treated with E₂ and oligoamines.