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. 2013 Feb 27;33(9):4165–4180. doi: 10.1523/JNEUROSCI.4185-12.2013

Figure 2.

Figure 2.

Tbr2 expression is ablated in cortical hem-derived Cajal-Retzius cells in Nestin-Cre;Tbr2flox/flox mice. A–A3, At E14.5, the majority of p73+ Cajal-Retzius cells in the cortical hem (CH) coexpress Tbr2 (arrows). B–B3, p73 expression is maintained in the CH of Nestin-Cre;Tbr2flox/flox mice at E14.5; however, Tbr2 protein is absent from these cells (B2, B3). C–D1, Similarly, Tbr2+ cells coexpress Reelin in the CH of control mice at E14.5, whereas Tbr2 protein is absent from Reelin+ Cajal-Retzius cells in Nestin-Cre;Tbr2flox/flox mice (D1). Regardless, the density of Cajal-Retzius cells in the CH of Nestin-Cre;Tbr2flox/flox mice appears approximately equivalent to controls at E14.5, as evidenced by Reelin (D1) and p73 staining (B3). E–F3, Likewise, proliferation of Cajal-Retzius cells in the CH appears unaffected by ablation of Tbr2, as an approximately equivalent number of p73+ cells coexpress PCNA (arrows) in controls (E1–E3) and Nestin-Cre;Tbr2flox/flox mice (F1–F3). Scale bars: A, 150 μm; A1, 50 μm; C, 100 μm; C1, 30 μm; E, 100 μm; E1, 15 μm. Regions delineated by dashed white boxes are shown in higher magnification in their respective adjacent panels.