Figure 2. Fungal SM recruitment inhibition screen.

(A) Schematic of the screening principle to identify secondary metabolites inducing neutrophil migration inhibition. (B) Example of microscope image resulting from a migration experiment with the outlines produced by the automated counting algorithm. Scale bar = 300 µm (C) Screening of A. nidulans and A. fumigatus purified secondary metabolites for human primary neutrophil migration inhibition properties. Displayed are the number of recruited neutrophils to a mix of fMLP and 10 µM concentration of compound normalized to the positive fMLP control. Compounds identified in red significantly reduce migration towards fMLP (p<0.05). 2-NH₂EM = 2 amine emodin, 2-OHEM = 2 hydroxyl emodin, 8-OHEM = 8 hydroxyl emodin, CT = control. (D) Compounds selected for in vivo screening.