Figure 4.

NTD-CTD CA mutant cores undergo accelerated uncoatingin vitro. Purified HIV-1 cores were diluted in STE buffer and incubated at 37°C for the indicated times. After pelleting the particles, the extent of uncoating was determined by quantifying CA levels in the pellets and supernatants. Note that variations in uncoating can occur from batch-to-batch isolated cores; hence for each panel analyzed, cores were isolated at the same time. Shown are the mean values of duplicate determinations, with error bars representing the range of values. The results are representative of a minimum of two independent experiments for each mutant.