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NTD-CTD CA mutants with altered capsid stability are impaired in reverse transcription in target cells. HeLa-P4 cells were inoculated with equal quantities of WT or mutant viruses. Cultures were harvested 8 hr. after infection and DNA isolated and assayed for second-strand transfer products by qPCR. Shown are the mean values and error bars represent the standard deviations from three independent experiments.
