Table 1.
Summary of viral mutant phenotypes
| Virus | Mutation Location | Single-cycle infectivity (% of WT ± SD) | Replication in CEM cells@ | % of mature cores# | Presence of cones/tubes | % core-associated CA (±SD) | Disassembly in vitro | Abro-gation of restriction | Reverse transcription in cells (% of WT ± SD) |
|---|---|---|---|---|---|---|---|---|---|
| WT |
- |
100 |
+++ |
50 |
+ |
14 ± 2 |
Normal |
Yes |
100 |
| H62A |
Helix 4 |
1.0 ± 0.3 |
- |
10 |
- |
1.1 ± 0.4 |
ND |
No |
1.3 ± 0.5 |
| Q63A |
Helix 4 |
27 ± 3 |
+ |
15 |
+ |
5.2 ± 0.2 |
Accelerated |
Yes |
50 ± 14 |
| A64D |
Helix 4 |
0 ± 0.1 |
- |
35 |
- |
1 ± 0.6 |
ND |
No |
1.3 ± 0.5 |
| M68A |
Helix 4 |
1.0 ± 1 |
- |
10 |
- |
0.8 ± 0.3 |
ND |
No |
1.3 ± 0.5 |
| E71A |
Helix 4 |
18 ± 4 |
- |
20 |
+ |
6.3 ± 2 |
Normal |
Yes |
49 ± 6.2 |
| E75A |
Helix 4 |
13 ± 3 |
+ |
20 |
+ |
3.6 ± 1 |
Accelerated |
Yes |
34 ± 6.2 |
| K140A |
Helix 7 |
1.0 ± 0 |
- |
20 |
- |
0 ± 0 |
ND |
No |
1.0 ± 0.8 |
| R162A |
Helix 8 |
0 ± 0.2 |
- |
35 |
+ |
0.2 ± 0.1 |
ND |
No |
1.7 ± 0.5 |
| V165A |
Helix 8 |
1.0 ± 1 |
- |
25 |
a |
31 ± 4 |
Slowed |
Yes |
2.0 ± 0.8 |
| D166A |
Helix 8 |
1.0 ± 1 |
- |
35 |
- |
1 ± 0.1 |
ND |
No |
1.3 ± 0.5 |
| R167A |
Helix 8 |
11 ± 2 |
+ |
35 |
+ |
6.4 ± 1.1 |
Accelerated |
No |
32 ± 5.2 |
| Q176A |
Helix 8 |
25 ± 3 |
++ |
30 |
+ |
8.1 ± 2 |
Normal |
Yes |
83 ± 13 |
| Q179A |
Helix 8 |
62 ± 12 |
+++ |
35 |
+ |
13 ± 2 |
Normal |
Yes |
94 ± 7.9 |
| E180A |
Helix 8 |
4 ± 1 |
- |
20 |
- |
5.1 ± 1.4 |
Normal |
Yes |
30 ± 1.7 |
| M215A | Helix 11 | 0 ± 0.3 | - | 20 | - | 0.5 ± 0.1 | ND | No | 1.5 ± 0.5 |
@ +++, replicates like wild type; ++, 3 days delayed; +, 15–18 days delayed; -, no replication within 30 days.
# mature cores exhibit an electron-dense core region, which is either bar-shaped, conical or spherical.
ND; not determined.
a: conical but morphologically aberrant cores.