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. 2013 Feb 27;12:21. doi: 10.1186/1475-2859-12-21

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Copyright © 2013 Collins et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of the flask volume to culture medium volume ratio on biomass and SELP production. Comparison of maximum SELP-59-A volumetric (blue) and specific (grey) productivities as well as maximum biomass levels measured (red) as a function of the flask volume to medium volume ratio. The maximum biomass values represent the highest dry cell weights (DCW, g/L) measured over the course of the experiment. The SELP-59-A volumetric and specific productivities were estimated from comparisons of band intensities on a sodium dodecyl sulphate polyacrylamide gel with a band of known concentration.