Figure 8.

Plasmid stability, without induction and with induction at various elapsed fermentation times (EFTs). Cultures induced during the exponential (2 hours EFT, biomass DCW of approx. 1 g/L, red), declining exponential (4 hours EFT, biomass DCW of approx. 2 g/L, green) and early stationary (8 hours EFT, biomass DCW of approx. 4 g/L, blue) phases of growth as well as non-induced cultures (black) are compared. The induction points (EFT) are indicated by arrows. The optimised shake flask conditions for SELP production as developed in the present study were used.