Table 1.
Details of the media investigated
| Medium | Preparation |
|---|---|
| Lysogeny broth (LB) |
10 g/L bacto tryptone, 5 g/L yeast extract, 5 g/L NaCl |
| Lysogeny broth-Miller (LBM) |
10 g/L bacto tryptone, 5 g/L yeast extract, 10 g/L NaCl, 0.98 g/L MgSO4 |
| Terrific broth (TB) |
5 g/L glycerol, 12 g/L bacto tryptone, 24 g/L yeast extract, 2.31 g/L KH2PO4 and 12.54 g/L K2HPO4 |
| Terrific broth with lactose (TBlac) |
5 g/L glycerol, 12 g/L bacto tryptone, 24 g/L yeast extract, 2.31 g/L KH2PO4 and 12.54 g/L K2HPO4, 2 g/L lactose |
| Modified terrific broth (TBmod) |
0.5 g/L glucose, 12 g/L bacto tryptone, 24 g/L yeast extract, 6.8 g/L KH2PO4, 7.1 g/L Na2HPO4.12H2O, 0.15 g/L MgSO4, 3.3 g/L (NH4)2SO4 |
| Terrific broth auto-induction medium (TBaim) |
0.5 g/L glucose, 2 g/L lactose, 12 g/L bacto tryptone, 24 g/L yeast extract, 6.8 g/L KH2PO4, 7.1 g/L Na2HPO4.12H2O, 0.15 g/L MgSO4 3.3 g/L (NH4)2SO4 |
| Super broth (SB) |
5 g/l glycerol, 35 g/L bacto tryptone, 20 g/L yeast extract, 2.31 g/L KH2PO4 and 12.54 g/L K2HPO4 |
| Modified super broth (SBmod) |
0.5 g/L glucose, 35 g/L bacto tryptone, 20 g/L yeast extract, 6.8 g/L KH2PO4, 7.1 g/L Na2HPO4.12H20, 0.15 g/L MgSO4, 3.3 g/L (NH4)2SO4 |
| Super broth auto-induction medium (SBaim) |
0.5 g/L glucose, 2 g/L lactose, 35 g/L bacto tryptone, 20 g/L yeast extract, 6.8 g/L KH2PO4, 7.1 g/L Na2HPO4.12H2O, 3.3 g/L (NH4)2SO4; 0.15 g/L MgSO4 |
| Super broth enriched (SBenrich) |
20g/l fructose, 32 g/L bacto tryptone, 60 g/L yeast extract, 5 g/L NaCl |
| Modified super optimal broth (SOC) |
4 g/L glucose, 20 g/L tryptone peptone, 5 g/L yeast extract, 0.58 g/l NaCl, 0.186 g/L KCl, 2.46 g/L MgSO4, 2 g/L MgCl2 |
| New Brunswick Scientific medium (NBS) |
25 g/L glucose, 5 g/L yeast extract, 2 g/L KH2PO4, 3 g/L K2HPO4, 0.5 g/L MgSO4.7H2O, 5 g/L (NH4)2HPO4, 1 mg/L thiamine, 3 mL/L Holmes trace elements solution [46] |
| Modified New Brunswick Scientific medium (NBSmod) |
5 g/L glycerol, 5 g/L yeast extract, 2 g/L KH2PO4, 3 g/L K2HPO4, 0.5 g/L MgSO4.7H2O, 5 g/L (NH4)2HPO4, 1 mg/L thiamine, 3 mL/L Holmes trace elements solution [46] |
| ZYB (ZYB) |
10 g/L NZ-amine, 5 g/L yeast extract, 5 g/L NaCl |
| ZYB buffered (ZYBbuff) |
10 g/L NZ-amine, 5 g/L yeast extract, 2.31 g/L KH2PO4, 12.54 g/L K2HPO4, 5 g/L NaCl |
| Minimal medium M9 (M9) |
10 g/L glucose, 0.85 g/L Na2HPO4.12H2O; 1 g/L NH4Cl; 3 g/L KH2PO4; 0.5 g/L NaCl; 0.24 g/L MgSO4 |
| Riesenberg minimal medium (Ries) | 20 g/L glucose, 13.3 g/L KH2PO4, 4 g/L (NH4)3PO4, 1.7 g/L citric acid, 0.24 g/L MgSO4, 3 mL Holmes trace element solution [46] |
All media were adjusted to pH 7.0 and sterilised by autoclaving at 121°C for 20 minutes, components such as phosphates and magnesium were filter sterilized and added separately after sterilisation. All media were supplemented with filter sterilised ampicillin at a final concentration of 200 μg/mL following sterilisation.