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Development of one-shot sample handling protocol. (a). Ambient storage conditions impair the pS6 status over time. Y-axis refers to % of pS6 baseline signals. (b). The one-shot sample handling protocol provided well-preserved pS6 signals for 24 hrs. (c). An overview of the phospho flow-based PD study proposed for clinical trials. Data shown (a-b) are representative of at least three independent studies.
