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. 2013 Apr 11;8(4):e60919. doi: 10.1371/journal.pone.0060919

Figure 2. SDF-1α increases tumor growth by directly acting on tumor cells.

Figure 2

A. Experimental design. B. Pretreatment of 4T1 cells with wound fluid derived from WT animals but not wound fluid derived from BALB/c nu/nu animals increased tumor growth in vivo as compared to pretreatment of 4T1 cells with plasma. mean ±95% CI, p<0.0001, n = 15 animals/group, ANOVA/Bonferroni’s Multiple Comparison Test, observation time: 18d. C. Pre-treatment of 4T1 cells with SDF-1α and plasma in vitro resulted in increased tumor growth in vivo as compared to pretreatment of 4T1 cells with plasma only. p = 0.0015, n = 15 animals/group, unpaired t-test, observation time: 22d, mean ±95% CI. D. Inhibition of SDF-1α/CXCR4 signaling with AMD3100 (AMD) during pre-treatment of 4T1 cells with wound fluid abolished increased tumor growth observed after pretreatment of 4T1 cells with wound fluid. p = 0.0016, n = 15 animals/group, ANOVA/Bonferroni’s Multiple Comparison Test, observation time: 22d, mean ±95% CI.