Table 7.
Concentration of GABA-related metabolic products and GABA-related gene expression
| nmol/g | ||
|---|---|---|
| Control | 2 mM Agmatine | |
| Putrescine | 20.3 | 39.8 |
| 4-aminobutyrate (GABA) | 110.2 | 191.7 |
| Glutamate | 1,616.1 | 2,930.7 |
| Gene ID (mips)a | Fold change | Description |
| FGSG_01572.2 | 0.4 | Glutamate decarboxylase |
| FGSG_05446.2 | 29.4 | Agmatinase |
| FGSG_06751.2 | 11.2 | 4-aminobutyrate aminotransferase |
| FGSG_04196.2 | 12.3 | Succinate-semialdehyde dehydrogenase |
The concentration of each substance was calculated from an analytical curve based on the peak area. Values are means of three independent experiments (n = 3)
Genes classified as induced or reduced and each metabolic compounds were those passing a sample t-test (P < 0.05)
aThe given gene IDs are entry numbers from the F. graminearum genome annotation FG3 by the Fusarium comparative sequencing project, Broad Institute of Harvard and MIT (http://www.broadinstitute.org/)