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. Author manuscript; available in PMC: 2013 Apr 12.
Published in final edited form as: Nat Commun. 2011 Jul 5;2:380. doi: 10.1038/ncomms1386

Figure 1. Identification of the putative sigma 2 receptor binding site as PGRMC1 using a novel photoaffinity probe.

Figure 1

a, WC-21 contains a FITC group and a photoactive azide (N3) moiety, and displays high sigma-2 receptor affinity and selectivity; Ki = 8.7 ± 1 nM for sigma-2 receptor and Ki > 4,000 nM for sigma-1 receptor. b, Following UV cross-linking, ligand-labeled proteins were enriched and separated through SDS-PAGE; WC-21 cross-linked proteins can be visualized using an anti-FITC antibody; SS is silver staining and WB is western blot. c, Proteomic studies of the FITC positive band identified two proteins which shared the same genetic name: progesterone receptor membrane component 1 (PGRMC1). The protein structure shows the peptides (bold) detected by mass spectrometry as well as the Cytochrome b5 domain (underlined).