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. Author manuscript; available in PMC: 2014 Mar 21.
Published in final edited form as: Chem Biol. 2013 Feb 21;20(3):403–415. doi: 10.1016/j.chembiol.2012.11.014

Figure 2.

Figure 2

LIMP-2 is an important GC proteostasis trafficking network component. (A) The knockdown of LIMP-2 significantly decreases the WT and N370S GC activity in the fibroblasts when compared to the NT siRNA control. (B and C) Silencing LIMP-2 diminishes the increase in L444P GC activity (B) and the endo H resistant post-ER GC glycoform in western blot analysis (C) that is afforded by either MG-132 or diltiazem alone. Quantification of endo H resistant GC bands is shown at the bottom of (C).(D and E) The modest transient overexpression of LIMP-2 in L444P GC fibroblasts increases the endo H resistant post-ER GC glycoform in western blot analysis (D) and the lysosomal trafficking of L444P GC in indirect immunofluorescence study (E). Quantification of endo H resistant GC bands and LIMP-2 bands is shown on the right of (D).The experiments were done three times. The data in (A)-(D) are reported as mean ± SEM (n=8 for A and B) and any statistical significance was calculated using a two-tailed Student's t-Test. * p < 0.05. See also Figure S2.