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Production and analysis of the SD1 human Fc fusion protein. (A) SDS-PAGE analysis of purified SD1-hFc (4 μg of protein per lane). NR: non-reducing; R: reducing; (B) Immunoprecipitation of endogenous mesothelin protein in A431/H9 (16), NCI-H226 and KMBC cell extracts. SD1 was used to pull down endogenous mesothelin protein in the cell lysate. C: an irrelevant VH single-domain human Fc fusion; IP: immunoprecipitation; Input: western blot on whole cell lysates before immunoprecipitation.
