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Binding properties of SD1-hFc. (A) Direct ELISA. MSLN: full-length human mesothelin protein (MSLN); mMSLN: mouse mesothelin. (B) Competition ELISA. (C) The dissociation equilibrium KD of SD1-hFc to the human mesothelin protein was 13.58 nM and (D) 16.08 nM for the peptide. (E, F) Recombinant mesothelin protein bound to MUC16 in the presence of SD1.
