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. Author manuscript; available in PMC: 2014 Feb 26.
Published in final edited form as: Biochemistry. 2013 Feb 14;52(8):1419–1428. doi: 10.1021/bi301651n

Figure 7.

Figure 7

X-band CW-EPR spectra of the cryotrapped cob(II)alamin-substrate radical pair in EAL in different protein samples. From top, spectra shown are as follows: WT: EALH6 protein. EutB+EutC: EAL enzyme reconstituted from purified EutB and EutC subunits. EutB: Purified EutB subunit, alone. EutC: Purified EutC subunit, alone. Arrows show g = 2.35 (g⊥ for cob(II)alamin) and g=2.0023 (free electron g-value). Experimental conditions: microwave frequency, 9.458 GHz; temperature, 120 K; microwave power, 2.04 mW; magnetic field modulation, 10.0 G; modulation frequency, 100 kHz; scan rate, 71 G/s; time constant, 10.24 ms.