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. 2013 Apr 12;4:88. doi: 10.3389/fpls.2013.00088

FIGURE 2.

FIGURE 2

Salicylic acid-induced ArabidopsisNIMIN1 and NIMIN2 are expressed differentially from each other and from PR-1. RNA samples were isolated from Arabidopsis seedlings or Arabidopsis leaves and analyzed as described in Figure 1A. Expression of NIMIN1 and NIMIN2 is compared to expression of NIMIN3 and PR-1. (A) RT-PCR analyses of RNAs from wild-type (Col-0) and npr1-1 and npr1-2 mutant seedlings. 1-1, npr1-1; 1-2, npr1-2. (B) RT-PCR analyses of RNAs from leaf tissue at different times after spraying plants with 1 mM SA. (C) Time course of SA-induced GUS reporter enzyme activities and PR-1 protein accumulation in tobacco seedlings transformed with NIMIN1Pro::GUS or NIMIN2Pro::GUS. Expression from the two NIMIN promoters is compared to reporter gene expression from the Nt -1533PR-1a promoter. For immunodetection of endogenous PR-1 proteins, equal amounts of protein were loaded in each lane of the SDS gels. Seedlings (T1 generation) were grown on selective medium with 0.3 mM SA. Similar results were obtained with independent lines of NIMIN1Pro::GUS, NIMIN2Pro::GUS and -1533PR-1aPro::GUS.