Fig 2.
Generation of ACOT7 brain-specific knockout mice. (A) Gene targeting strategy. (B) Southern blot of ES cell clones showing correct targeting. (C) Western blot for mice with a brain-specific knockout of ACOT7. (D) Representative electron micrograph images of control and Acot7N−/− brains from fed mice. pre, presynaptic; post, postsynaptic; nuc, nucleus; mito, mitochondria. (E and F) Total long-chain acyl-CoA thioesterase (E) or total long-chain acyl-CoA synthetase (F) activity in homogenates from 3- to 4-month-old male and female control and Acot7N−/− brains; n = 4 to 6. (G and H) Hypothalamic long-chain acyl-CoA content (G) or brain coenzyme A content (H) from 3- to 4-month-old male and female control and Acot7N−/− mice; n = 6. Data represent averages ± standard errors of the means.