Fig 4.
ACOT7 inhibits fasting-induced lipid metabolism. (A to D) Brain nonesterified fatty acids (A), phospholipids (B), TAG (C), or cholesterol content (D) in lipid fractions derived from 3- to 4-month-old male and female control and Acot7N−/− brains with or without overnight fasting; n = 6 to 8. (E) Cytosolic phospholipase A2 activity in 3- to 4-month-old male and female control and Acot7N−/− brains with or without overnight fasting; n = 6. (F to I) Oleate oxidation to CO2 and acid-stable metabolites (ASM) (F), glucose oxidation (G), oleate incorporation into lipids (H), or acetate incorporation into lipids (I) in brain slices derived from overnight-fasted 3- to 6-month-old male and female control and Acot7N−/− mice; n = 6 to 8. White bars represent data from control mice, and black bars represent data from Acot7N−/− mice, except in panel F (Con, control; N-/-, Acot7N−/−; Cnts, counts). Data represent averages ± standard errors of the means. “∗” indicates P ≤ 0.05 by two-tailed Student's t test comparing controls to Acot7N−/− mice.