Fig 2.

Analyses of Yju2 N and C domains. (A) Two-hybrid interactions of Yju2 domains with Ntc90 and Ntc77. The N and C domains of Yju2 and full-length Yju2 were fused to the GAL4-activation domain (AD), and Ntc77 and Nt90 were fused to the GAL4-DNA binding domain (BD). The interactions were assayed in terms of the activation of ADE2 under the control of the GAL2 promoter. FL, full-length; C, C domain; N, N domain. (B) Growth analysis. TC2 cells were transformed with pRS414 and pRS415 vectors alone (−), with pRS414.YJU2 and pRS415 (FL), with pRS414.YJU2-N and pRS415 (N), with pRS414.YJU2-C and pRS415 (C), or with pRS414.YJU2-N and pRS415.YJU2-C (N+C). Cells were cultured in raffinose-containing synthetic complete medium and then spotted onto galactose- or glucose-containing plates in 10-fold serial dilutions. FL, full-length; N, N domain; C, C domain; N+C, combination of N and C domains.