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. 2013 May;33(9):1746–1755. doi: 10.1128/MCB.00035-13

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Fig 3 — Complementation of Yju2-depleted extracts. (A) Splicing reactions were carried out for 30 min in mock-depleted (lane 1) or Yju2-depleted extracts without addition (lane 2) or with the addition of 0.3 μM Yju2-FL (lane 3), 1.5 μM Yju2-N (lane 4), 0.4 μM Yju2-C (lane 5), or a combination of 1.5 μM Yju2-N and 0.4 μM Yju2-C (lane 6). (B) Splicing was carried out for 30 min in mock-depleted (lane 1) or Yju2-depleted extracts without addition (lane 2) or with the addition of 0.3 μM Yju2-FL (lane 3), 0.4 μM Yju2-C (lane 4), or 0.4 μM (lanes 5 and 9), 0.8 μM (lanes 6 and 10), 1.5 μM (lanes 7 and 11), or 3.0 μM (lanes 8 and 12) Yju2-N without Yju2-C addition (lanes 5 to 8) or with the addition of 0.4 μM Yju2-C (lanes 9 to 12). (C) Splicing was carried out in Yju2-depleted extracts with the addition of 0.2 μM Yju2-FL (lanes 1 to 7) or Yju2-N (lanes 8 to 14) for 0 to 30 min. dYju2, Yju2 depletion; M, mock treatment; FL, full length; N, Yju2-N; C, Yju2-C.